The experiment was conducted using the scientific method of making observation. Today you will be working with the aquatic plant, elodea, also known as anacharis. Five distinct species of elodea are recognized, all native to the new world bowmen et al. Yasser bio1031001 9192016 abstract the objective of the elodea leaf experiment was to examine the process called cyclosis. Sprig of elodea distilled water w baking soda test tube or clear glass timer or watch. To release the energy contained in the bonds of glucose, the. You will be comparing the effects of two solutions. The materials required for this experiment for each group of the students are. In the first part, you will use the weight of pieces of potato to see how much water moves in and out of cells in different salt solutions. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. Label lines must be horizontal or vertical drawn with a ruler 5. Elodea, they are asked to design an experiment which will allow them to demonstrate the use of. In this activity, you will use the scientific method of inquiry to perform a laboratory experiment. Prepare a wet mount of an elodea leaf with aquarium water.
Elodea is a submersed aquatic plant within the hydrocharitaceae or waterleaf family. Photosynthesis in elodea lab essay example graduateway. Using forceps remove a small leaf from the tip of an elodea plant and lay. Carolinas solution preparation manual carolina caresheet. Photosynthesis elodea lab photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. Observing photosynthesis this is a very simple lab that allows students to observe the formation of oxygen bubble during photosynthesis. In the second part, you will observe plasmolysis in an aquatic plant, elodea.
Elodea canadensis or canadian waterweed is native to temperate north america, originally. Write a hypothesis to explain which color light will be best for photosynthesis and why. Observe the cells under normal conditions, and make a sketch of what you see. As the slide warms from the light of the microscope, you may see the chloroplasts moving, a process called cytoplasmic streaming. Pdf the oxygen transfer capacity of submerged plant elodea. This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater. Bromthymol blue is used as an indicator for the presence of co2 in solution. This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater plant elodea. While observing the leaf under the microscope, wick a solution of 6% nacl sodium chloride across the slide. Elodea cornell institute for biology teachers cornell blogs. Right picture shows the boaters nightmare, a tangled mat of brazilian elodea. Start with the low power objective and work your way until you have focused the leaf using the high power objective. An older name for this genus is anacharis, which serves as a common name.
Initial setup and data collection will take one lab period one hour and. To do so, the plant must use carbon dioxide and water in a process called photosynthesis. As a result of class analysis of the experiments, students will conclude that. The elodea plant is a very helpful pond plant that helps to reduce algae, clarify water and provide shade for fish. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Brazilian elodea, egeria densa, anacharis, philotria densa, giant elodea, brazilian waterweed hannah darrin fish 423. How is the amount of bubbles of oxygen released by the elodea rate of photosynthesis effected by the distance to the light. Qualitative and quantitative plasmolysis lab do not write. Elodea lab this lab is designed to show how photosynthesis takes place in the aquatic plant elodea. Elodea, genus of five or six species of submerged aquatic plants in the frogsbit family hydrocharitaceae, useful in aquariums and in laboratory demonstrations of cellular activities. The cylinder will be filled with normal tap water or spring water, and the stem of the elodea should be submerged 12 inches in the water. Shortly before the lab starts, add carbon dioxide to the bromothymol blue.
Carbon cycle lab photosynthesis and respiration welcome. Elodea stem with leaves x x x aluminum foil to block light x x 10. Photosynthesis virtual labs northern highlands regional. Brine shrimp animals and elodea plants will change the levels of. Pdf there are insufficient data for the development of process design. Cellular respiration virtual lab carbon transfer through snails and elodea background.
Controlling the rate of photosynthesis life science teaching. Live elodea anacharis aquatic plant for biology and life science is healthy, viable and ready for transplant into your freshwater aquarium or for laboratory study. Water passes through cell membranes from areas of high water concentration to areas of lower water concentration. Qualitative and quantitative plasmolysis lab do not write on this lab return it at the end of the class period. This plant can commonly be found in lake habitats and you may be more familiar with it if you have an aquarium. All organisms are dependent on a healthy carbon dioxideoxygen balance. Maintain your margins no marks and drawn with a ruler 2. As photosynthesis takes place, the co 2 would be used up, making the solution turn towards blue. Plasmolysis purpose in this lab you will be looking at how different solutions affect elodea and the structure of onion cells. Brazilian elodea, egeria densa, anacharis, philotria densa. Turn each tube upside down to maximize light exposure and place the rack of inverted tubes directly underneath the lamp at your lab bench. Students will begin by writing the equation for photosynthesis, which has been. Cyclosis is the movement of the flow of cytosol which is the liquid component of the cytoplasm found within a plant or animal cell.
Elodea canadensis, elodea nuttallii and elodea callitrichoides. Select carbon transfer through snails and elodea from the list of labs. The glucose made by plants is used by plants and animals as a source of energy. Biology lab manual in the presence of all the things necessary for photosynthesis to take place, the level of co 2 would be high so the solution should be yellow. Teaching plant pigments with elodea leaves penguin bay. Elodea high school inquiryscientific method plants. Cellular respiration virtual lab currituck county schools. Carbon transfer through snails and elodea virtual lab. Arto kurtto fi, editor inchief of atlas florae europaeae, naturhistoriska. We did this by using a gilson respirometer and samples of elodea plant placed in h2o and nahco 3 solutionsboth of which were operated under lighte d and darkened external conditionsto. Photosynthesis and respiration in elodea teacher section. Determining nacl concentration of plasmolysis 9272012 dr.
Elodea plants are native to the new world, though a number of species have established themselves as invasive species in ponds and quiet waterways. The color changed because the carbondioxide from our breath went into the solution making it green. Record the following information in your lab journal. Elodea is a genus of 6 species of aquatic plants often called the waterweeds described as a genus in 1803. Photosynthesis and cellular respiration are key processes in maintaining this balance. You will need to provide slides of cheek cells, onion cells, elodea, and volvox. Prepare a wet mount of one leaf from the water plant elodea using the water in which it is kept. Make sure to write your protocol, concentrations, etc in your lab notebook.
The purpose for using this plant is to study the internal structure of a leaf cell, more. Bromothymol blue is an indicator solution to those used to test for food nutrients. Elodea is considered an invasive species in some areas and care must be exercised when disposing of left over plants. Demonstrate that environmental co2 is used during photosynthesis in elodea. Using forceps remove a small leaf from the tip of an elodea plant and lay it flat in the drop of water. Elodea plant for sale live aquarium plants petsmart. Create a graph comparing the ph values over time and write a brief.
Kayla 21019 f honors biology elodea lab report experimental design sheet. Which side of a penny will hold more drops of water before spilling over on to a paper towel. Classified in the frogsbit family hydrocharitaceae, elodea is native to the americas and is also widely used as aquarium vegetation and laboratory demonstrations of cellular activities. For optimum results of your investigations, we recommend transferring them to water and putting them under lights for 2 days before using them. Photosynthesis and respiration in elodea experimental design sheet restate the two tasks of your experiment as hypotheses. The positive control group represents the orange liquid because we are representing and testing environmental co2s role in. Lab if you intend to use elodea in a laboratory investigation, you can use the plants immediately after rinsing them. Your lab group is asked to design, execute, and analyze an experiment that tries to accomplish the following two tasks. Record the starting time on your worksheet, then turn on the lamp and allow the experiment to run for two hours.
Click link for tuesday and wednesday lab this should take you to virtual biology labs. This lab can be done as a demonstration to begin the unit, or it can done as an inquiry lab where students try to devise an explanation for the. They draw and label what they observe under high power. To do this, use a soaked qtip to place a drop of aquarium water towards one end of the slide. Devotional but without faith it is impossible to please him.
My group and i measured photosynthesis and respiration rates of the single celled protist algae elodea. Describe the shape and the location of chloroplasts. It can grow when floating freely or rooted in substrate. In this lab activity you will observe the effects of osmosis on plant cells. Gather one elodea leaf from the teacher and make a wet mount slide of the elodea. Because the leaves of elodea are only a few cells thick, they will be easy to observe under the microscope to look at cells and cell parts. With a permanent marker or wax pencil, write your initials or other mark to. It starts as a blue solution in water, and turns yellow in the presence of carbon dioxide.
View a prepared slide of elodea anacharis, which is an aquarium plant. A neat lab sheet introduces cell biologists to the general structure of both animal and plant cells. In this lab, you will be experimenting with how different variables affect the rate of photosynthesis. Pdf plasmolysis in elodea lab victoria newton academia. Students will place the elodea sprig upside down into the graduated cylinder. As elodea uses carbon dioxide from the initial solution, a change in ph occurs, causing a color. Before and after finishing the second experiment, the cod, n total, and. If you dont have an aquarium and need to dispose of elodea make sure you completely dry it off before putting it in the trash or on a compost pile.
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